2D3Q

Crystal Structure of a Decolorizing Peroxidase (DyP) That Catalyses the Biological Oxidation of Anthraquinone Derivatives

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	95
Detector technology	CCD
Collection date	2005-02-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.7
Spacegroup name	P 65 2 2
Unit cell lengths	136.212, 136.212, 363.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.265
Rwork	0.234
R-free	0.26700
Structure solution method	MIR
RMSD bond length	0.020
RMSD bond angle	1.190
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	89434
Completeness [%]	94.1	68.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.2	283	0.89M (NH4)2SO4, 0.92M NaCl, pH 4.2, SMALL TUBES, temperature 283K
1	SMALL TUBES	4.2	283	0.89M (NH4)2SO4, 0.92M NaCl, pH 4.2, SMALL TUBES, temperature 283K