2D3B
Crystal Structure of the Maize Glutamine Synthetase complexed with AMPPNP and Methionine sulfoximine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-14 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.881, 190.943, 117.904 |
| Unit cell angles | 90.00, 101.23, 90.00 |
Refinement procedure
| Resolution | 33.500 - 3.500 |
| R-factor | 0.16804 |
| Rwork | 0.166 |
| R-free | 0.20905 |
| Structure solution method | MIR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.616 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.084 | 0.259 |
| Number of reflections | 41598 | |
| <I/σ(I)> | 8.81 | 2.32 |
| Completeness [%] | 75.6 | 78.9 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 9% PEG8000, 5% MPD, 100MM Tris-HCl, 10MM MnCl2, 1MM AMPPNP, 1MM L-Methionine sulfoximine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
