2D3A
Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.795, 191.041, 118.103 |
| Unit cell angles | 90.00, 101.47, 90.00 |
Refinement procedure
| Resolution | 26.130 - 2.630 |
| R-factor | 0.18593 |
| Rwork | 0.184 |
| R-free | 0.21950 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.064 | 0.385 |
| Number of reflections | 107420 | |
| <I/σ(I)> | 15.82 | 2.08 |
| Completeness [%] | 87.1 | 65.3 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 9% PEG8000, 5% MPD, 100MM Tris-HCl, 10MM MnCl2, 1MM ATP, 1MM L-Methionine sulfoximine , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
