2D33
Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 325.226, 325.226, 105.181 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.16356 |
| Rwork | 0.162 |
| R-free | 0.19116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4g |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.096 | 0.304 |
| Number of reflections | 127490 | |
| <I/σ(I)> | 6.2 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium formate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
