2D2I
Crystal Structure of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp. complexed with Nadp+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.006, 79.588, 206.155 |
Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.500 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.960 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.067 | 0.265 |
Number of reflections | 71057 | |
<I/σ(I)> | 7.7 | 2.8 |
Completeness [%] | 85.8 | 85.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | ammonium sulfate, potassium sodium tartrate, citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |