2D1C
Crystal Structure Of TT0538 protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-15 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.022, 95.184, 72.989 |
| Unit cell angles | 90.00, 92.07, 90.00 |
Refinement procedure
| Resolution | 36.490 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.260 |
| Number of reflections | 92029 | |
| <I/σ(I)> | 9.5 | 4.3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 295 | NH2SO4, PEG 4000, pH 5.6, MICROBATCH, temperature 295.0K |
| 1 | MICROBATCH | 5.6 | 295 | NH2SO4, PEG 4000, pH 5.6, MICROBATCH, temperature 295.0K |
| 1 | MICROBATCH | 5.6 | 295 | NH2SO4, PEG 4000, pH 5.6, MICROBATCH, temperature 295.0K |
