2CZU

lipocalin-type prostaglandin D synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-23
Detector	RIGAKU RAXIS V
Wavelength(s)	1.010
Spacegroup name	P 21 21 21
Unit cell lengths	46.200, 66.800, 105.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.800 - 2.100
R-factor	0.24509
Rwork	0.243
R-free	0.27932
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	C2221 MAD model
RMSD bond length	0.006
RMSD bond angle	1.480
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5.0)

Data quality characteristics

	Outer shell
Low resolution limit [Å]	56.800
High resolution limit [Å]	2.100
Completeness [%]	90.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295.5	malonate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K