2CXE
Crystal structure of octameric ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Pyrococcus horikoshii OT3 (form-2 crystal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-17 |
Detector | ADSC QUANTUM |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.478, 149.207, 107.461 |
Unit cell angles | 90.00, 127.37, 90.00 |
Refinement procedure
Resolution | 46.720 - 3.000 |
R-factor | 0.295 |
Rwork | 0.295 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.074 |
RMSD bond angle | 5.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 39367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | magnesium chloride, sodium chloride, sodium acetate, PEG6000, Tris, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |