2CWA
Crystal Structure Of The Single-stranded DNA Binding Protein From Thermus Thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-29 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.90000, 0.97905, 0.97940 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 91.560, 91.560, 132.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.650 - 1.960 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.28200 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.650 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.058 | 0.319 |
Number of reflections | 24054 | |
<I/σ(I)> | 24.3 | 7.2 |
Completeness [%] | 99.2 | 96 |
Redundancy | 18.99 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | Sodium Nitrate, Tris-HCl, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |