2CV5
Crystal structure of human nucleosome core particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.564, 108.370, 169.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.224 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 61700 | |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MANGANESE (II) ION | ||
| 2 | 1 | 1 | CHLORIDE ION | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | MANGANESE (II) ION | ||
| 5 | 1 | 2 | CHLORIDE ION | ||
| 6 | 1 | 2 | H2O |
