2CNX

WDR5 and Histone H3 Lysine 4 dimethyl complex at 2.1 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	100.249, 46.753, 64.789
Unit cell angles	90.00, 107.08, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.1918
Rwork	0.192
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1erj
RMSD bond length	0.006
RMSD bond angle	1.598
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.240
Number of reflections	16605
<I/σ(I)>	27.2	5.1
Completeness [%]	98.5	97
Redundancy	6.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1