2CNW
GDPALF4 complex of the SRP GTPases Ffh and FtsY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 188.686, 188.686, 44.588 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.680 - 2.390 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1okk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.285 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.900 | 2.520 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 287923 | |
| <I/σ(I)> | 11.7 | 3.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS-HCL (PH 8.5), 25% (W/V) PEG8000 |
