2CMT
The structure of reduced cyclophilin A from s. mansoni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2006-04-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.654, 59.213, 61.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.800 - 1.500 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zmf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.985 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.020 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.170 |
| Number of reflections | 23994 | |
| <I/σ(I)> | 7.21 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.6 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M IMIDAZOLE/MALATE PH 5.5, 20 % PEG 600 |
