2CKI
Structure of Ulilysin, a member of the pappalysin family of metzincin metalloendopeptidases.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.564, 126.148, 87.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.000 - 1.700 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.24100 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.286 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 61061 | |
<I/σ(I)> | 11.6 | 3.4 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | EQUIVOLUMETRIC SITTING DROPS CONSISTING OF PROTEIN (5 MG PER ML IN 30MM TRIS PH 7.5, 2MM DTT, 100MM NACL) AND RESERVOIR SOLUTION (18% PEG 8000, 0.1M MES PH6.5, 0.2M CACL2) AFTER SEVERAL WEEKS AT 20 DEGREES CELCIUS. |