2CKI
Structure of Ulilysin, a member of the pappalysin family of metzincin metalloendopeptidases.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.564, 126.148, 87.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 61061 | |
| <I/σ(I)> | 11.6 | 3.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | EQUIVOLUMETRIC SITTING DROPS CONSISTING OF PROTEIN (5 MG PER ML IN 30MM TRIS PH 7.5, 2MM DTT, 100MM NACL) AND RESERVOIR SOLUTION (18% PEG 8000, 0.1M MES PH6.5, 0.2M CACL2) AFTER SEVERAL WEEKS AT 20 DEGREES CELCIUS. |
