2CK2
Structure of core-swapped mutant of fibronectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Collection date | 2006-02-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.703, 37.227, 137.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.550 - 2.000 |
| R-factor | 0.2008 |
| Rwork | 0.201 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ten |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.550 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.230 |
| Number of reflections | 12995 | |
| <I/σ(I)> | 23 | 8.9 |
| Completeness [%] | 97.3 | 95.9 |
| Redundancy | 8.9 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 50% PEG-400, 0.1 M ACTEATE, 0.2 M LITHIUM SULPHATE, PH 5.1 |
