2CIJ

membrane-bound glutamate carboxypeptidase II (GCPII) with bound methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	260
Detector technology	CCD
Collection date	2004-01-27
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	102.692, 130.777, 160.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.174
Rwork	0.172
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c6g
RMSD bond length	0.017
RMSD bond angle	1.753
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.080
Number of reflections	42562
<I/σ(I)>	18.2	3.9
Completeness [%]	99.9	99.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.25		pH 7.25