2CG8
The bifunctional dihydroneopterin aldolase 6-hydroxymethyl-7,8- dihydropterin synthase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 149.440, 149.440, 238.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 127.000 - 2.900 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbu |
| RMSD bond length | 0.040 |
| RMSD bond angle | 3.164 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.970 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 62341 | |
| <I/σ(I)> | 9.4 | 4.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6 | 5.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.00 |
