2CFV
Crystal structure of human protein tyrosine phosphatase receptor type J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-09 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 86.060, 86.060, 119.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.250 - 2.500 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ahs |
RMSD bond length | 0.012 |
RMSD bond angle | 1.296 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.270 |
Number of reflections | 11491 | |
<I/σ(I)> | 15.25 | 4.18 |
Completeness [%] | 98.8 | 91.2 |
Redundancy | 3.76 | 3.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 0.01M NICL2,0.1M TRIS PH 8.5, 1M LI2SO4,150 UL SITTING DROPS |