2CFM
ATP-DEPENDENT DNA LIGASE FROM PYROCOCCUS FURIOSUS
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-21 |
Detector | RIGAKU RAXIS-IV |
Wavelength(s) | 0.9797, 0.9791, 0.9787, 0.9587 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.866, 87.399, 62.648 |
Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.320 |
Number of reflections | 56984 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.4 | 98.5 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ETHANOL, PH 8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |