2CF8
Complex of recombinant human thrombin with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-28 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.070, 71.510, 72.400 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 71.250 - 1.300 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.19900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1oyt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.040 | 0.300 |
| Number of reflections | 85919 | |
| <I/σ(I)> | 17.8 | 4.2 |
| Completeness [%] | 98.2 | 95.9 |
| Redundancy | 3.58 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | PH 7.40 |
