2CE9

A WRPW peptide bound to the Groucho-TLE WD40 domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-07-22
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	107.810, 56.478, 126.642
Unit cell angles	90.00, 112.68, 90.00

Refinement procedure

Resolution	117.040 - 2.120
R-factor	0.181
Rwork	0.178
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gxr
RMSD bond length	0.020
RMSD bond angle	2.012
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.240
High resolution limit [Å]	2.120	2.120
Rmerge	0.060	0.250
Number of reflections	78005
<I/σ(I)>	13.4	4.6
Completeness [%]	97.5	83
Redundancy	3.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	287	6MG/ML PROTEIN WAS MIXED 1:1 WITH 12% PEG8000, 100MM NACACODYLATE PH6.5, 100MM NAACETATE AT 14C IN MICROBATCH. PEPTIDES WERE ADDED TO THE PROTEIN CRYSTALS IN 125 MM NACL, 25 MM TRIS PH8.0, 0.5 MM EDTA TO A FINAL CONCENTRATION OF 0.76 MM, AND INCUBATED FOR 16 H BEFORE HARVESTING AND WASHING IN 50MM NA CACODYLATE PH6.5, 12 % PEG 8K, AND CRYO-PROTECTED IN 30% ETHYLENE GLYCOL 50MM NA CACODYLATE PH6.5, 12 % PEG 8K, pH 6.50