2CE7
EDTA treated
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 165.092, 165.092, 235.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.440 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.510 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 120494 | |
| <I/σ(I)> | 14.1 | 2.9 |
| Completeness [%] | 99.1 | 97.6 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 30% PEG400, 0.2M CACL2, 0.1 M HEPES PH 7.5 |
