2CE2

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-20
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	49.000, 53.900, 116.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.000
R-factor	0.145
Rwork	0.144
R-free	0.16300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q21
RMSD bond length	0.012
RMSD bond angle	1.719
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.000
High resolution limit [Å]	0.990	0.990
Rmerge	0.050	0.600
Number of reflections	82821
<I/σ(I)>	14.96	2.33
Completeness [%]	97.0	96.7
Redundancy	4.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	PROTEIN SOLUTION: 17.22 MG/ML PROTEIN, 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE, 35% PEG 400 MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K