2CDE
Structure and binding kinetics of three different human CD1d-alpha- Galactosylceramide specific T cell receptors - iNKT-TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-02 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 289.360, 84.950, 78.860 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.283 |
| Rwork | 0.283 |
| R-free | 0.35040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mi5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.180 | 0.880 |
| Number of reflections | 23622 | |
| <I/σ(I)> | 6.4 | 1.4 |
| Completeness [%] | 98.1 | 90.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5 M NACL, 11 % PEG 8000, 50 MM HEPES PH7.0 |
