2CDA
Sulfolobus solfataricus Glucose Dehydrogenase 1 in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.620, 92.296, 138.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 138.680 - 2.280 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.817 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.200 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.110 | 0.260 |
| Number of reflections | 38690 | |
| <I/σ(I)> | 12.8 | 6.3 |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 % PEG 8000, 0.1 MM TRIS (PH 8.0), 4.5 % PROPAN-2-OL |
