2CB3

Crystal structure of peptidoglycan recognition protein-LE in complex with tracheal cytotoxin (monomeric diaminopimelic acid-type peptidoglycan)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 2 3
Unit cell lengths	217.530, 217.530, 217.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.1985
Rwork	0.199
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oht
RMSD bond length	0.006
RMSD bond angle	1.305
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.200
Number of reflections	66354
<I/σ(I)>	24.3	4.9
Completeness [%]	92.9	90.4
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1