2C7W
Crystal Structure of human vascular endothelial growth factor-B: Identification of amino acids important for angiogeninc activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 64 |
| Unit cell lengths | 120.806, 120.806, 39.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.480 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.096 | 0.630 |
| Number of reflections | 12054 | |
| <I/σ(I)> | 12.9 | 2.4 |
| Completeness [%] | 97.4 | 99.3 |
| Redundancy | 9.1 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.1M HEPES (PH 6.8), 0.5M AMMONIUM SULPHATE 50%, MPD |
