2C7N

Human Rabex-5 residues 1-74 in complex with Ubiquitin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-16
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	44.300, 68.900, 98.500
Unit cell angles	108.20, 102.70, 90.40

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.198
Rwork	0.195
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.020 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL2Map
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.033 *	0.169 *
Number of reflections	57954
<I/σ(I)>	12.7	3.5
Completeness [%]	92.3	66.4
Redundancy	2.7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	20 *	SITTING DROP 300NL PLUS 300NL 0.2M AMMONIUM ACETATE 0.1M NACITRATE PH 6.5 25% PEG400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	PEG4000	25 (%)