2C74

14-3-3 Protein Eta (Human) Complexed to Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	75.731, 75.360, 113.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.990 - 2.700
R-factor	0.224
Rwork	0.220
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bq0
RMSD bond length	0.014
RMSD bond angle	1.391
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.480
Number of reflections	18419
<I/σ(I)>	12	2.4
Completeness [%]	99.8	99.9
Redundancy	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		0.1M CITRATE PH 5.6, 20% ISOPROPANOL, 20% PEG4000