2C6Y

Crystal structure of interleukin enhancer-binding factor 1 bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	58.736, 58.736, 324.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.440 - 2.400
R-factor	0.233
Rwork	0.233
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.440	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.530
Number of reflections	12691
<I/σ(I)>	45.1	3.8
Completeness [%]	99.2	98.1
Redundancy	8.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1