2C63
14-3-3 Protein Eta (Human) Complexed to Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.251, 79.398, 125.109 |
| Unit cell angles | 90.00, 95.15, 90.00 |
Refinement procedure
| Resolution | 125.000 - 2.150 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bq0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.467 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.960 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 61914 | |
| <I/σ(I)> | 12 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M MGCL2, 0.1M HEPES PH 7.5, 25% PEG 3350 |
