2C60
crystal structure of human mitogen-activated protein kinase kinase kinase 3 isoform 2 phox domain at 1.25 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-22 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.800, 42.300, 30.600 |
Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
Resolution | 39.720 - 1.250 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.18200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.455 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.320 | 1.350 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.070 | 0.260 |
Number of reflections | 26421 | |
<I/σ(I)> | 15.81 | 5.25 |
Completeness [%] | 93.8 | 88.1 |
Redundancy | 5.78 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 150 NANOLITRE SITTING DROPS, 0.2 KSCN, 20% PEG3350, 10% ETHYLENE GLYCOL |