2C57
H.pylori type II dehydroquinase in complex with FA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 103.863, 103.863, 217.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c4w |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.076 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 3.150 |
| High resolution limit [Å] | 3.080 | 3.100 |
| Rmerge | 0.090 | 0.850 |
| Number of reflections | 43781 | |
| <I/σ(I)> | 10 | 1.2 |
| Completeness [%] | 90.4 | 81.6 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 10MG/ML PROTEIN AGAINST 30% 1,4 BUTANDIOL, 0.1M SODIUM ACETATE PH 4.5 USING SITTING DROP METHOD |
