2C4M
Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.266, 187.620, 129.315 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 306185 | |
| <I/σ(I)> | 5.1 | 3.1 |
| Completeness [%] | 89.7 | 75.9 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | HANGING DROP VAPOUR DIFFUSION 8.4MG/ML PROTEIN SOLUTION, 0.1M SODIUM ACETATE PH 5.0, 8% PEG 8,000, 0.2M SODIUM FORMATE |
