2C46
CRYSTAL STRUCTURE OF THE HUMAN RNA guanylyltransferase and 5'- phosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.400, 161.600, 60.700 |
Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
Resolution | 80.850 - 1.600 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9s |
RMSD bond length | 0.011 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.400 |
Number of reflections | 133485 | |
<I/σ(I)> | 10.29 | 2.71 |
Completeness [%] | 90.4 | 87.9 |
Redundancy | 3.79 | 2.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2 M POTASSIUM CITRATE |