2C43
STRUCTURE OF AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE- PHOSPHOPANTETHEINYL TRANSFERASE IN COMPLEX WITH COENZYME A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-29 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.589, 68.957, 70.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.930 |
| R-factor | 0.162 |
| Rwork | 0.158 |
| R-free | 0.21900 |
| Structure solution method | MIR |
| Starting model (for MR) | 2byd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.790 | 2.020 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 19791 | |
| <I/σ(I)> | 16.53 | 3.59 |
| Completeness [%] | 80.3 | 29.2 |
| Redundancy | 5.04 | 0.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG6K 2.0 M NACL |
