2C3Z
Crystal structure of a truncated variant of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.200, 61.400, 80.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| Structure solution method | MAD |
| Starting model (for MR) | 1igs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.874 |
| Data reduction software | MOSFLM |
| Data scaling software | ROTAVATA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 5474 | |
| <I/σ(I)> | 5.6 | 2.2 |
| Completeness [%] | 96.4 | 92.8 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG8000 0.2 M AMMONIUM SULFATE 0.1 M MES, PH 6.5 |
