2C32
Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D-crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-09 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 188.271, 188.271, 188.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 7.010 |
| R-factor | 0.39 |
| Rwork | 0.390 |
| R-free | 0.38700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ymg |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 7.380 |
| High resolution limit [Å] | 7.010 | 7.010 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 900 | |
| <I/σ(I)> | 5.21 | 2.06 |
| Completeness [%] | 89.9 | 100 |
| Redundancy | 6.69 | 6.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.9 | 100 MM BICINE NAOH PH 8.9, 350 MM NACL, 34 % (V/V) PEG400 |
