2C23
14-3-3 Protein Beta (Human) in complex with exoenzyme S peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-03 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.908, 86.841, 121.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.860 - 2.650 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bq0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 9558 | |
| <I/σ(I)> | 13.4 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.05M MGCL2,0.1M HEPES PH7.5, 30% PEG MME 550, pH 8.00 |
