2C20
CRYSTAL STRUCTURE OF UDP-GLUCOSE 4-EPIMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 136.165, 136.165, 248.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.020 - 2.700 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gy8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 70638 | |
| <I/σ(I)> | 16 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22% PEG 2K MME, 0.2M CALCIUM ACETATE, 0.1 MES PH 6.5 |
