1GY8

Trypanosoma brucei UDP-galactose 4' epimerase

Summary for 1GY8

• Entry DOI: 10.2210/pdb1gy8/pdb
• Descriptor: UDP-GALACTOSE 4-EPIMERASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, URIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: epimerase, galactose, trypanosoma brucei, oxidoreductase
• Biological source: TRYPANOSOMA BRUCEI
• Total number of polymer chains: 4
• Total formula weight: 179496.53

Authors

Shaw, M.P.,Bond, C.S.,Hunter, W.N. (deposition date: 2002-04-21, release date: 2003-05-22, Last modification date: 2023-12-13)

Primary citation

Shaw, M.P.,Bond, C.S.,Roper, J.R.,Gourley, D.G.,Ferguson, M.A.J.,Hunter, W.N.
High-Resolution Crystal Structure of Trypanosoma Brucei Udp-Galactose 4'-Epimerase: A Potential Target for Structure-Based Development of Novel Trypanocides
Mol.Biochem.Parasitol., 126:173-, 2003

PubMed Abstract: The crystal structure of UDP-galactose 4'-epimerase from the protozoan parasite Trypanosoma brucei in complex with the cofactor NAD(+) and a fragment of the substrates, UDP, has been determined at 2.0 A resolution (1 A = 0.1 nm). This enzyme, recently proven to be essential for this pathogenic parasite, shares 33% sequence identity with the corresponding enzyme in the human host. Structural comparisons indicate that many of the protein-ligand interactions are conserved between the two enzymes. However, in the UDP-binding pocket there is a non-conservative substitution from Gly237 in the human enzyme to Cys266 in the T. brucei enzyme. Such a significant difference could be exploited by the structure-based design of selective inhibitors using the structure of the trypanosomatid enzyme as a template.

PubMed: 12615316
DOI: 10.1016/S0166-6851(02)00243-8

Experimental method

X-RAY DIFFRACTION (2 Å)