2C1Z
Structure and activity of a flavonoid 3-O glucosyltransferase reveals the basis for plant natural product modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-05-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.106, 93.531, 106.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.780 - 1.900 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.23200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.373 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.780 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 36495 | |
<I/σ(I)> | 13 | 2.8 |
Completeness [%] | 99.0 | 98 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% PEG 10000, 0.1M BISTRIS-PROPANE PH 7.0, 0.5% PLUCORONIC F-68 |