2C1H
The X-ray Structure of Chlorobium vibrioforme 5-Aminolaevulinic Acid Dehydratase Complexed with a Diacid Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 30 |
| Detector technology | CCD |
| Collection date | 2004-10-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 126.480, 126.480, 81.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.440 - 2.600 |
| R-factor | 0.263 |
| Rwork | 0.260 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w1z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.034 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.490 |
| Number of reflections | 24586 | |
| <I/σ(I)> | 5.6 | 1.6 |
| Completeness [%] | 91.6 | 94.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
