2BXS
Human Monoamine Oxidase A in complex with Clorgyline, Crystal Form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.374, 152.076, 82.203 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.150 |
| R-factor | 0.271 |
| Rwork | 0.268 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2q |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.740 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.010 | 0.040 |
| Number of reflections | 27718 | |
| Completeness [%] | 91.3 | 67.1 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 5% PEG 6000, 100 MM NACITRATE, 100 MM LISULPHATE, 50 MM KPI PH 7.0. |
