2BWJ
Structure of adenylate kinase 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-13 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 180.375, 180.375, 121.701 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21300 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.348 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.150 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.090 | 0.370 |
Number of reflections | 7913 | |
<I/σ(I)> | 12.53 | 2.35 |
Completeness [%] | 95.0 | 63.1 |
Redundancy | 8.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M HEPES PH7.5, 0.1M NACL, 1.6M (NH4)2SO4, pH 7.50 |