2BWG
Structure of human guanosine monophosphate reductase GMPR1 in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-31 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.312, 114.418, 115.129 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eep |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.352 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.370 |
| Number of reflections | 154258 | |
| <I/σ(I)> | 7.14 | 2 |
| Completeness [%] | 90.1 | 80.8 |
| Redundancy | 2.83 | 2.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% PEG3350 0.30 M K3CIT |
