2BW0
Crystal Structure of the hydrolase domain of Human 10-Formyltetrahydrofolate 2 dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2005-06-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.226, 64.502, 58.891 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 41.670 - 1.700 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3i |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.621 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 167067 | |
| <I/σ(I)> | 4.5 | 1.9 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.7M AMMONIUM SULPLATE 50MM HEPES PH 7.8 |
