2BUJ
Crystal structure of the human Serine-threonine Kinase 16 in complex with staurosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-23 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.554, 168.901, 129.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.820 - 2.600 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.536 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.800 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.590 |
Number of reflections | 35446 | |
<I/σ(I)> | 13.52 | 2.29 |
Completeness [%] | 99.3 | 94.3 |
Redundancy | 7.83 | 5.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 200 NL SITTING DROP, 1.1 AMMONIUM SULFATE, 1% PEG3350, 0.1 M BIS-TRIS PH 5.0 |