2BPV
HIV-1 protease-inhibitor complex
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.800, 87.380, 46.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.980 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.059 * |
| Total number of observations | 90150 * |
| Number of reflections | 16262 * |
| Completeness [%] | 83.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 6 (mg/ml) | |
| 2 | 1 | drop | MES | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | 3 (mM) | ||
| 6 | 1 | reservoir | 0.6 (M) | ||
| 7 | 1 | reservoir | 0.1 (M) |
