2BOI
1.1A Structure of Chromobacterium Violaceum Lectin CV2L in Complex with alpha-methyl-fucoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.093, 89.836, 46.479 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.470 - 1.100 |
R-factor | 0.102 |
Rwork | 0.101 |
R-free | 0.11700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gzt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.634 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | ACORN |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.380 | 1.130 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.070 | 0.300 |
Number of reflections | 87549 | |
<I/σ(I)> | 5.36 | 2.34 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.14 | 7.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PEG 8000 10%, 0.1 M (NH4)2SO4 PH 5 |